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stem cell factor  (Miltenyi Biotec)


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    Miltenyi Biotec stem cell factor
    Stem Cell Factor, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 52 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/stem+cell+factor/Human+SCF%2C+research+grade/bio_rxiv__64898__2026__03__19__712939-413-19-22
    Average 95 stars, based on 52 article reviews
    stem cell factor - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Cell Culture:

    Article Title: Oncodevelopmental plasticity of the skeleton in myeloid neoplasms
    Article Snippet: Mesoderm and hematopoietic specification was induced by supplementation with 10 ng/mL FGF2 (PeproTech, #100-18B), 10 ng/mL BMP4 (Miltenyi Biotec, #130-111-168), and 10μM Y-27632 ROCK inhibitor (Abcam, #129830-38-2). .. From day 2 until 14, EBs were cultured with daily partial medium exchange and stage-specific cytokine supplementation, including 1× stem cell factor (Miltenyi Biotec, #130-096-692), 25 ng/mL VEGF (Miltenyi Biotec, #130-109-385), 10 ng/mL FLT3L (PeproTech, #300-19-250), 30 ng/mL IL-3 (ImmunoTools, #11340037), 25 ng/mL hyper–IL-6 (Stem Cell Technologies, #78050) and 50ng/mL M-CSF (Thermo Fischer, #300-25). ..

    Article Title: The Parkinson’s disease-associated LRRK2-G2019S variant restricts serine metabolism, leading to microglial inflammation and dopaminergic neuron degeneration
    Article Snippet: Briefly, 4 × 10 6 iPSCs were seeded into AggreWellTM 800 plate pre-treated with Anti-Adherence Rinsing Solution (Stemcell Technologies, 34860). .. Cells were cultured for 4 days in embryoid body (EB) medium, consisting of Essential 8 Basal medium supplemented with ROCK inhibitor, 50 ng/ml bone morphogenetic protein 4 (BMP4, Biolegend, 795606), 50 ng/ml vascular endothelial growth factor 165 (VEGF-165, Biolegend, 583708), and 20 ng/ml stem cell factor (SCF, Miltenyi Biotec, 130–096–695). .. EBs were harvested and transferred to ultra-low attachment 6-well plates (ThermoFisher Scientific, 07–200–601) and cultured for an additional 3 days in EB medium.

    Article Title: Interleukin-33 mediated regulation of microRNAs in human cord blood-derived mast cells: Implications for infection, immunity, and inflammation
    Article Snippet: CD34 + hematopoietic stem cells (HSCs) were isolated using Lymph prep (1.077 g/ml; Axis Shield, Oslo, Norway), followed by CD34 microbead labeling and magnetic activated cell sorting (Miltenyi Biotec Inc., Bergisch Gladbach, Germany). .. CD34 + HSCs were cultured in AIM-V medium (Thermo Fisher Scientific, Waltham, MA, USA) supplemented with recombinant human interleukin-6 (rhIL-6, 50 ng/mL, Thermo Fisher Scientific) and stem cell factor (rhSCF, 100ng/mL, Miltenyi Biotec Inc.) for 8–10 weeks to support their differentiation into hCBMCs. .. Phenotyping was accomplished using flow cytometry, cell imaging, and gene set enrichment analysis as previously reported [ ]. hCBMCs were activated with either 10 or 20 ng/mL recombinant human IL-33 (rhIL-33; Sino Biological, Beijing, China) and incubated for 6 or 24 h to observe immediate and prolonged responses as previously described [ , ].

    Generated:

    Article Title: Assessing Aβ‐independent effects of Module 42 on immune function in vitro
    Article Snippet: .. Embryoid bodies (EBs) were generated by seeding 4 × 10 6 hiPSCs per well in AggreWell‐800 plates (34811, STEMCELL Technologies) and maintained in mTeSRTM1 medium supplemented with (Bone morphogenetic protein‐4; 50 ng/mL, PHC9534, Life Technologies), (Vascular endothelial growth factor; 50 ng/mL, PHC9391, Life Technologies), and (Stem cell factor; 20 ng/mL, 130‐096‐695, Miltenyi Biotec) for 3 days. .. EBs were subsequently transferred to low‐attachment plates (657970, Greiner Bio‐One) and maintained for another 3 days.

    Incubation:

    Article Title: Senataxin promotes recombination fidelity during antigen receptor gene diversification.
    Article Snippet: Antigen receptor diversity depends on the assembly of variable (V), diverse (D), and joining (J) exons in genes encoding immunoglobulins (Igs) and T cell receptors (TCRs).. During V(D)J recombination, DNA doublestrand breaks (DSBs) introduced by the RAG1/2 nuclease complex are repaired by the process of nonhomologous endjoining (NHEJ).. We hypothesized that functional redundancies between NHEJ and the chromatin DSB response, which depends on the kinase ATM, potentially masked the activity of additional factors that regulate V(D)J recombination.

    Recombinant:

    Article Title: Interleukin-33 mediated regulation of microRNAs in human cord blood-derived mast cells: Implications for infection, immunity, and inflammation
    Article Snippet: CD34 + hematopoietic stem cells (HSCs) were isolated using Lymph prep (1.077 g/ml; Axis Shield, Oslo, Norway), followed by CD34 microbead labeling and magnetic activated cell sorting (Miltenyi Biotec Inc., Bergisch Gladbach, Germany). .. CD34 + HSCs were cultured in AIM-V medium (Thermo Fisher Scientific, Waltham, MA, USA) supplemented with recombinant human interleukin-6 (rhIL-6, 50 ng/mL, Thermo Fisher Scientific) and stem cell factor (rhSCF, 100ng/mL, Miltenyi Biotec Inc.) for 8–10 weeks to support their differentiation into hCBMCs. .. Phenotyping was accomplished using flow cytometry, cell imaging, and gene set enrichment analysis as previously reported [ ]. hCBMCs were activated with either 10 or 20 ng/mL recombinant human IL-33 (rhIL-33; Sino Biological, Beijing, China) and incubated for 6 or 24 h to observe immediate and prolonged responses as previously described [ , ].



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